The BNP-led 20-party alliance has again called a 72-hour countrywide hartal from 6am on Sunday.
BNP Joint Secretary General Salahuddin Ahmed in a statement on Saturday made the announcement.
After ending hartal during five working days last week alongside nonstop roads, waterways and railways blockade, the alliance declared hartal again.
The statement said the strike was called to press home for the alliance's demand of a fresh polls under a nutral caretaker government, establishing people's voting rights and democracy and release of arrested leaders and activists.
Killings of and torturing on the leaders and activists of the alliance parties, Interference in judiciary, controlling the media and confinement of Khaleda zia at her Gulshan office were also protested in the statement.
The alliance earlier enforced hartal from February 1 to February 5.